1. OBJECTIVE
In this experiment, you will learn about the main morphologies of fungi. Additionally, the experiment develops the ability to identify macroscopically and microscopically the probable etiological agents of mycoses.


At the end of this experiment, you should be able to:

Identify a filamentous fungus;

identify the structure of a yeast-like fungus;

process different types of fungi (filamentous and yeast-like);

learn to report the visualized result.

2. Where to use these concepts?
Knowing how to identify the different fungal structures is a prerequisite for developing experimental skills and abilities that enable the diagnosis of mycoses. Furthermore, the correct identification of the etiological agent allows for more effective treatment for the individual affected by mycosis.


3. The experiment
This experiment will use the following inputs: bench disinfection kit (almotolia or pisseta, one with 70% alcohol and the other with 1% sodium hypochlorite), Sabouraud agar, 40% potassium hydroxide, Gram stain kit and other instruments that They will serve to assist in carrying out the practice, such as an “L” shaped fungus handle, slides in 70% alcohol, coverslips in 70% alcohol, Pasteur pipette, wax pencil and blue or black pilot.


4. Security
In this practice, gloves, a mask and a lab coat, also called a dust jacket, will be used. Although the practice is considered low risk for the student, these three protective equipment are essential for a safe laboratory environment and are in accordance with Good Laboratory Practices. The gloves will prevent any possible contamination with agents harmful to the skin, the mask protects against possible contaminated aerosols and the lab coat protects the body as a whole.


5. Scenario
The experiment environment features a Bunsen burner on the workbench, as well as supplies and instruments. You must select and use them to ensure the correct execution of the experiments.