Experiment Objectives:
Manipulate different laboratory equipment.
Prepare fecal samples using the Hoffmann method (spontaneous sedimentation).
Make microscopy slides with the sediment.
Detect helminth eggs and larvae and protozoan cysts.
Practical Applications:
The Hoffmann technique is essential for researching helminth eggs and larvae and protozoan cysts in fresh or preserved samples, increasing the sensitivity of parasite detection.
Experiment Step by Step:
Prepare fecal material using the Hoffmann method.
Analyze the sediment under an optical microscope to identify parasitic forms.
Safety first:
Follow biosafety level 2 (NB2) rules to avoid contamination.
Wear a lab coat, latex gloves, mask and safety glasses.
Experiment Scenario:
Work in a clinical parasitology laboratory with horizontal benches.
Use organized instruments and equipment, including microscopes and slides.