Hill House is a mansion in a location that is never specified but is between many hills. The story concerns four main characters: Dr. John Montague, an investigator of the supernatural; Eleanor Vance, a shy young woman who resents having lived as a recluse caring for her demanding disabled mother;[4] Theodora, a flamboyant, bohemian artist and Luke Sanderson, the young heir to Hill House, who is host to the others.

Dr. Montague hopes to find scientific evidence of the existence of the supernatural. He rents Hill House for a summer and invites as his guests several people whom he has chosen because of their experiences with paranormal events. Of these, only Eleanor and Theodora accept. Eleanor travels to the house, where she and Theodora will live in isolation with Montague and Luke.